RBS

Part:BBa_M36070

Designed by: Alex Rosay, Justin Diep, Ben Blankenmeister   Group: Stanford BIOE44 - S11   (2012-12-07)

Bicistronic RBS - BIOFAB ID: apFAB681 (BCD1)

Ribosome Binding Site with high strength based on bicistronic junction architecture. Facilitates translation of genes at reliably high rates. Information from BIOFAB: Strain performance: 435.43 Strain performance SD: 53.55.


Usage and Biology

Bicistronic Ribosome Binding Sites have shown to provide ~93% chance to obtain expected gene expression within a two-fold target level. This provides improved control over reliable expression by decreasing engineering error by ~750%(Mutalik et al.).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None